Method and Errors of Human Cell Line Authentication DNA Tests
Cell lines play a vital role in various biological and genetic research and experimentations. They have transformed fields like drug development, cancer research, and genetics, providing consistent and replicable outcomes. Authenticating the cell lines being studied is also critical for the success of the research or the experiment. Such a DNA test is called a Cell Line Authentication Test and significantly contributes to scientific knowledge. This article will explore the significance and errors of Cell Line Authentication DNA Tests in India. Method Used for Authenticating the Cell Lines Cell line authentication depends on the analysis of Short Tandem Repeats (STRs). These STRs are repetitive sequences spanning 3-7 base pairs located throughout the DNA strand. These repetitive sequences are polymorphic. It means that the specific patterns of a locus or position can be replicated multiple times. The ATCC/ANSI/ASN-0002 process uses the S...