Ensure Research Accuracy with Cell Line Authentication Test in India

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Have you ever wondered if your cell line of interest is really what it seems to be? Maybe you're working on important experiments and need to make sure your cells haven't been contaminated or mixed up with other samples. These concerns are more common, especially when you’re working on important research. 

Therefore, a reliable cell line authentication test in India can provide the scientific proof you need to ensure your research results are accurate and trustworthy.

Why Does Cell Line Authentication Matter for Your Research?

Upon working on different cell lines in a lab, a significant amount of time, money, and efforts are invested. However, many researchers don't realize that cell line contamination and misidentification happen frequently in labs.

Studies show that a large percentage of cell lines used in research are either contaminated with other cells or completely misidentified. This means your months of hard work could be based on the wrong type of cells entirely. 

Common Issues that Affect the Cell Line Authentication

There are three types of common issues that affect the cell line authentication process: 

  1. Misidentification of Cell Lines - Sometimes, cell lines are misidentified during the discovery, and in some cases, they’re wrongly labeled. This further makes the research less accurate and unreliable.

  2. Genetic Drift - Cell lines stored in the lab for years have the tendency to shift their original identity. This also causes issues in the important lab-based research on certain medicines, vaccines, and diseases.

  3. Cross-Contamination of Cell Lines - This scenario happens when a cell line gets mixed with another cell line. Moreover, it’s sometimes caused due to bacteria and environmental contaminants. 

Process of Authenticating a Cell Line

Cell line authentication requires the following steps:

  1. Sample Collection

The first stage involves gathering a small amount of the DNA sample for examination, which can be obtained directly from the cell culture.

  1. DNA Isolation

This process involves extracting the DNA from the cells through specialized chemicals and methods. Then the DNA is prepared for further examination.

  1. DNA Multiplication

When the DNA is prepared, a procedure known as Polymerase Chain Reaction (PCR) is performed. PCR represents a method that creates multiple copies of particular DNA regions. In this instance, it targets Short Tandem Repeats (STRs), which are brief repetitive sequences within DNA that are different in each cell line.

  1. DNA Characterization

After the PCR, the amplified DNA undergoes analysis to establish a genetic profile for the cell line. This profile remains different for each cell line. 

  1. Final Outcome

It involves comparing the examined cell line's DNA profile against established, verified cell line profiles maintained in databases. When the profiles match, the cell line receives confirmation as authentic. Otherwise, it may indicate the sample is incorrectly identified, contaminated, or has genetically drifted.

Why Choose DNA Forensics Laboratory for Cell Line Authentication?

When selecting a laboratory for your authentication needs, look for labs like DNA Forensics Laboratory (DFL) that follow international standards and guidelines. Experts at DFL use extensive methods and maintain proper quality control procedures. Besides, DFL also provides DNA testing services for peace of mind, sponsored immigration, and legal disputes. 

For more information or to book a cell line authentication test in India, call us at +91 8010177771. Alternatively, you can also send a message on WhatsApp at +91 9266615552.

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