Basics of Cell Line Authentication and Where to Get it

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Cell line authentication is a vital research tool. It is essential in fields like medicine, cell biology, genetics, vaccine development, etc. STR (short tandem repeat) profiling is the preferred method for human cell line authentication. The STR analysis helps in detecting misidentified and cross-contaminated cells in a cell line. In addition, the technique compares multiple STR markers between multiple cellular genomes.

Why is Cell Line Authentication required?

Tissue culture cells are crucial for scientific research in many laboratories. However, many of these cells are misidentified or contaminated with other cells. Thus, cell line authentication is important to save the loss of money and time due to such misidentified or cross-contaminated cell lines. The process is now recommended by the National Institutes of Health and many journals. 

Cell Line Authentication is typically done:

•            Before starting new research or experiment

•            Before finalizing and publishing a research 

•            When it is becoming challenging to replicate the results of an experiment 

•            At frequent intervals

•            During cell line identification



What is a cell line?

A cell line is a generic terminology for a set of cells maintained in cell culture for an extensive time. It is a definite life and preserves its function and phenotype in the culture. Cell culture is maintained artificially to support cells in favorable conditions. 

Cell Line Authentication Test in India

A Cell Line Authentication Test in India depends on the sensitivity and significance of the research, and hence is a crucial decision to make. The test is done either every time a new cell line is received or at regular intervals. The process includes detecting genetically drifted, misidentified, or cross-contaminated cells that hinder scientific research. This is achieved by genetic profiling using polymorphic STR loci followed by its analysis. These STRs are repetitive sequences on the human genome. Standards recommend the use of at least eight STR loci for detailed analysis, which starts with amplification.

We perform the following steps in sequence for a Cell Line Authentication Test in India:

       1. STR profiling of the required cell lines.
2. Matching the profile against the reference database.
3. Using the Match Formulation technique to find out the percent match and check for cross-contamination.
4. Making a list of the misidentified cell lines.


DNA Forensics Laboratory Pvt. Ltd. is a NABL accredited lab that provides DNA testing services, including a Cell Line Authentication Test in India. We used standardized techniques for a cell line authentication test. Research bodies, pharmaceutical organizations, and biotechnology institutes in India use our Cell Line Authentication Test. Learn more about our Cell Line Authentication Test in India by calling +91 8010177771 or dropping a WhatsApp message at +91 9213177771. Our customer service representatives will guide you through the process, and you can also request to book the test to our representatives.


Location: SCF – 193, First Floor Modern Market, Mattaur Rd, Mattaur, Sector 70, Sahibzada Ajit Singh Nagar, Punjab 160071, India

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